INHIBITION OF CELLULAR INVASION AND INDUCTION OF ANOIKIS IN MOUSE MELANOMA CELLS BY AN ANTI-INFLAMMATORY AGENT DTCM-GLUTARIMIDE
https://doi.org/10.24060/2076-3093-2012-0-3-4-9
Abstract
Previously, we designed DTCM-glutarimide as a novel anti-inflammatory agent inhibiting macrophage activation. It also showed anti-inflammatory activity in vivo. In the present research, we evaluated the anti-metastatic activity of this agent using mouse melanoma B16-F10 cells. DTCM-glutarimide inhibited the cellular invasion in the Matrigel chamber assay, lowering matrix metalloproteinase 9 (MMP9) expression. It also induced anoikis rather than apoptosis in polyHEMA-coated plates. It increased Bax and decreased Bcl-XL expressions, and activated p53 lowereing the MDM2 expression. Thus, DTCM-glutarimide was found to inhibit cellular invasion and to induce anoikis in B16-F10 cells. It is non-toxic, and easily prepared, therefore, is considered to be a new candidate of anti-metastasis agent.
About the Authors
A. Kaneda
Keio University
Japan
Department of Applied Chemistry, Faculty of Science and Technology, Yokohama
Japan
Department of Applied Chemistry, Faculty of Science and Technology, Yokohama
Sh. Kh. Gantsev
Bashkir State Medical University; Scientific Research Institute of Oncology
Russian Federation
Russian Federation
K. Umezawa
Aichi Medical University
Japan
Japan
Department of Molecular Target Medicine Screening, School of Medicine, Nagakute
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Review
For citations:
Kaneda A., Gantsev Sh.Kh., Umezawa K. INHIBITION OF CELLULAR INVASION AND INDUCTION OF ANOIKIS IN MOUSE MELANOMA CELLS BY AN ANTI-INFLAMMATORY AGENT DTCM-GLUTARIMIDE. Creative surgery and oncology. 2012;(3):4-9. (In Russ.) https://doi.org/10.24060/2076-3093-2012-0-3-4-9
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